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HardyCHROM Urine Biplate (HUrBi), Separates Gram + and -, Chromogenic BiPlate, 10/pack

$76.95
For larger quantities: Request a Quote
Medex SKU:
HRD-J100
Packing Info:
10/Pack
Usually Ships:
3 - 5 Business Days

Description

Primary Uses

The J100 HUrBi™ plate is primarily designed for use in clinical microbiology laboratories to analyze urine specimens. Its main applications are:

  • Cultivation: To grow microorganisms (bacteria and yeast) that may be present in a patient's urine sample.

  • Enumeration: To perform a "colony count," which helps determine the quantity of microbes present. This is crucial for distinguishing between a true urinary tract infection (UTI) and simple contamination.

  • Differentiation: To quickly and visually distinguish between different types of bacteria and yeast that commonly cause UTIs. This helps in providing a preliminary identification of the pathogen.

While its main design is for urinary pathogens, it can also be used for other types of clinical specimens.


Key Features

The J100 biplate has several features that make it an effective tool for diagnosing UTIs:

  • Chromogenic Medium: This is its most important feature. The medium contains special chromogenic (color-producing) substrates. As different microbes grow, they release specific enzymes that break down these substrates, causing their colonies to turn distinct, easy-to-read colors (e.g., E. coli may appear pink/magenta, while Enterococcus appears turquoise blue).

  • Biplate Design (Selective): The plate is split into two separate sections, each containing a different type of selective medium:

    • Side I: Contains selective agents that encourage the growth of gram-positive organisms (like Enterococcus and Staphylococcus) and yeast (Candida) while inhibiting gram-negative bacteria.

    • Side II: Contains selective agents that encourage the growth of gram-negative organisms (like E. coli, Klebsiella, and Proteus) while inhibiting gram-positive bacteria.

  • Rapid Differentiation: The combination of the biplate's selectivity and the chromogenic "color-coding" allows technicians to:

    • Quickly determine if an infection is caused by gram-positive or gram-negative bacteria, or yeast.

    • Easily spot mixed infections (more than one type of microbe growing).

    • Presumptively identify common pathogens like E. coli and Enterococcus based on color alone, often without needing further tests.

  • Proteus Swarming Inhibition: The medium is formulated to inhibit the "swarming" motility of Proteus species. This is a significant feature, as swarming can cause the bacteria to spread over the entire plate, making it impossible to see or isolate other organisms.